Analysis of Amino Acids in Oxidized and Unoxidized Feed Samples (MA-374): Pickering Derivative
Introduction
- Commission Regulation (EC) No 152-2009 published in the Official Journal of the European Union lays down sampling and analytical methods for the official control of animal feed.
- This regulation describes analytical methods for the control of the composition of feed ingredients and compound feed products. The establishment of an amino acid profile is an important means of controlling the quality and nutritional value of animal feed.
- This regulation specifies HPLC with post-column derivatization with Ninhydrin reagent as the method for the analysis of total and free amino acids.
- The Pickering Laboratory has developed an analytical method to comply with all the chromatographic requirements of Commission Regulation (EC) No 152-2009. The same method is used for the analysis of oxidized and non-oxidized animal feed samples.
Method
Analytical Conditions
Column: High-efficiency Sodium cation-exchange column,
4.0 x 150 mm, Catalog Number 1154150T
Guard: Cation-exchange GARD™,
Catalog Number 1700-3102
Flow Rate: 0.4 mL/min
Mobile Phase: Na270, Na740, RG011. See method in Table 1
Injection Volume: 10 uL
Post-Column Conditions
Post-Column System: Onyx PCX or Pinnacle PCX
Reactor Volume: 0.5 mL
Reagent: Trione®
Column Temperature: See Method in Table 2
Reactor Temperature: 130 °C
Flow Rate: 0.25 mL/min
Detection: UV/VIS 570 nm for primary amino acids,
440 nm for secondary amino acidsCalibration
Analysis of amino acids in oxidized and unoxidized animal feed
Analysis of Amino Acids in Oxidized and Unoxidized Feed Samples (MA-374)
Amino acid analysis derivatization device or Pickering amino acid
Pickering Onyx PCX banner 2 amino acid post-column derivatization equipment
Contact for more information on Analysis of amino acids or amino acids in oxidized and non-oxidized animal feed: Zalo 0987445016
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